Comparative Genomic Hybridization (CGH) Service
CD Genomics is a world-leading company that can provide the best solutions for global customers in the field of microarrays. We have successfully completed much scientific research and non-clinical research projects to ensure that we provide the best products and services to customers all over the world.
Many defects in human development are caused by the increase or decrease of chromosomes and chromosome fragments that occur before or after fertilization, and DNA dose changes in somatic cells are a common cause of cancer. Tumor suppressor genes play an important role in tumor formation and can be detected by deletion and mutation analysis. The comparative genomic hybridization (CGH) developed in 1992 has changed the genome-wide analysis of cancer genetic variation, but due to the limitation of the resolution, the array-based CGH (aCGH) method was developed in 1995.
Fig.1 Schematic overview of the microarray-based comparative genomic hybridization technique. (Oostlander A E, et al. 2004)
There are many forms of genetic variation in the genome of eukaryotic cells, from abnormal chromosomal morphology and number to a large number of submicroscopic copy number changes in DNA fragments, and even single nucleotide changes. Broadly speaking, the variation of DNA fragments existing on chromosomes is copy number variation (CNV). CNV affects gene expression by destroying the original pattern of gene expression and changing the gene dosage. It can also indirectly affect gene expression through positional effects, phenotypic variation, and even disease.
aCGH Analysis Service
Traditional CGH provides a model for detecting CNV in cell and tissue samples. Combined with microarray technology, aCGH can detect tiny chromosomal imbalances, copy number variations (CNV), and accurately define their size and variant gene content.
| Sample Pretreatment |
Extract DNA from received tissues (fresh tissues and fixed tissue samples), cells, and blood samples. |
| Amplification |
Add random primers to the sample for amplification. |
| Labeling |
Label the reference sample and the test sample with fluorescent dyes. |
| Microarray Hybridization |
Use the previously determined microarray to hybridize with the labeled sample. |
| Scanning |
Use laser excitation and scan. |
| Data Processing and Analysis |
Determine the fluorescence ratio of the hybridization signal of the test sample and the reference sample and obtain the relative copy number information. |
Fig 2. Flow chart of aCGH analysis service of Bio-microarray.
Technical Advantages
High Efficiency. It can simultaneously detect the aneuploidy, deletion, duplication, and/or amplification of any locus represented on the array.
High Throughput. One measurement performed using this technology is equivalent to thousands of FISH experiments, thus saving labor and costs.
Wide Application. Study the structure of specific chromosomal regions (interstitial deletion (caused by two breaks of chromosome arms, loss of inserts and recombination of chromosome fragments)), various types of rearrangements in specific chromosomal regions (centrioles, centromeres).
CD Genomics provides CGH analysis services based on microarrays. We have a well-equipped experimental platform, a large and professional team of scientists, and we are committed to cooperating with researchers from all over the world to meet the needs of our customers. Customers can contact our employees directly and provide timely feedback on their queries. If you are interested in our one-stop solution service, please contact us for more detailed information.
References
- Oostlander A E, Meijer G A, Ylstra B. Microarray-based comparative genomic hybridization and its applications in human genetics[J]. Clinical genetics, 2004, 66(6): 488-495.
- Zhang C, Cerveira E, Romanovitch M, et al. Array-based comparative genomic hybridization (aCGH)[M]//Cancer Cytogenetics. Humana Press, New York, NY, 2017: 167-179.
- Holcomb I N, Trask B J. Comparative genomic hybridization to detect variation in the copy number of large DNA segments[J]. Cold Spring Harbor Protocols, 2011, 2011(11): pdb. top066589.
Our products and services are for research purposes ONLY. We do not perform any private testing services that are not for laboratory use.
