As a high-quality supplier of microarrays, CD Genomics is committed to providing a variety of useful microarray tools and professional testing services for scientific research and non-clinical research. Our microarray platform provides a full range of microarray-based mitochondrial non-coding RNA analysis services to help our global customers conduct related research and reduce research costs. With our time-tested capabilities and expertise, we are confident to provide you with quality services to escort your research.
Like nuclear DNA, mitochondrial DNA is also regulated by epigenetic factors, especially DNA methylation and non-coding RNA (ncRNA). This in turn gave birth to mitochondrial genetics. The mitochondrial genome is very small, but it also provides non-coding regions, such as the replacement loop (D-loop), which is responsible for controlling mtDNA replication and transcription. Compared with the nuclear genome, the mitochondrial genome is also 10-20 times more sensitive to molecular variation. The mechanism of action of mitochondrial small non-coding RNA is currently unclear. Long non-coding RNA (mt-lncRNA) may come from mtDNA transcription, and can regulate the stability and transcription of mt-mRNA, or can act as a bait for mitochondrial RNA binding protein (mtRBP). Polymeric transcription of mtDNA may lead to the formation of double-stranded RNA (mt-dsRNA). Under normal conditions, L-chain-derived transcripts will be rapidly degraded, thereby preventing the formation of mt-dsRNA. Abnormal degradation will cause mt-dsRNA to accumulate and be released into the cytoplasm. Once mt-dsRNA is released into the cytoplasm, it may participate in the response of some signals. Studies have shown that the nucleus and mitochondria constantly communicate with each other to regulate different cellular pathways, but little is known about the basic mechanism of mitochondrial genetics and nuclear-mitochondrial communication, as well as the relationship between ncRNA and mitochondrial function and related diseases. As a new participant in gene regulation, ncRNA has great research prospects.
Fig 1. Mitochondrial proteome and transcriptome. (Gusic M, et al. 2020)
We have designed a microarray tool for mt-ncRNA analysis. This tool can be used to analyze the role of non-coding RNA in mitochondrial epigenetics. The probe design is based on public non-coding sequence databases and published literature data. This tool can be used to analyze reported mitochondrial ncRNA and transcript sequences regulated by these ncRNAs, including some miRNAs, sRNAs, and lncRNAs involved in regulation. A unique partition design is adopted to realize the simultaneous detection of these types of ncRNAs on a microarray. The lncRNA probe set includes some lncRNAs that regulate the expression of oxidative phosphorylation (OXPHOS) complex nuclear coding subunits or participate in mitochondrial-induced apoptosis and some nuclear-coded lncRNAs that play an important role in mitochondrial energy balance. The level of mitochondrial activity in mammals can be regulated by mitochondrial and nuclear-encoded lncRNAs associated with cancer and inflammatory diseases. Our probe set also includes these lncRNAs that are located in mitochondria and regulate mitochondrial function and are associated with cancer or acute inflammation. In addition to covering these non-coding regions, the probe design also includes adjacent fragments that partially overlap with these sequences. We provide one-stop testing and analysis services, from sample pretreatment, microarray testing to data collection and analysis, you can trust us with samples.
Fig 2. The workflow of mitochondrial ncRNA microarray service.
CD Genomics provides high-quality microarray tools and testing services for mt-ncRNA research. We have a large and well-equipped team of scientists who are committed to cooperating with researchers from all over the world. If you have relevant testing requirements, please contact us for more detailed information.