CD Genomics is a leading global company who can provide the best solution for worldwide customers in the field of RNA microarray. We have successfully accomplished many projects in lncRNA microarray. We guarantee the finest results for our customers all over the world.
Long non-coding RNA (lncRNA) is a type of RNA with the transcript length exceeding 200 nt and not encoding proteins. In cells, the abundance of lncRNA accounts for 70% to 98%, and some lncRNA length can reach thousands of bp. Although lncRNA does not encode any protein, its expression is still specific in different tissues and developmental stages, indicating its biological significance. Most of the lncRNAs are located in the nucleus. Some of their corresponding DNA regions overlap with protein-coding genes, and some are located between genes or introns. Since lncRNAs seem to have little biological function, they were considered to be "evolutionary noise" and have no biological function. However, recent studies have shown that lncRNAs can regulate gene expression at epigenetic, transcriptional and post-transcriptional levels1,2. They are involved in many important regulatory processes, such as X-chromosome silencing, genomic imprinting, chromatin modification, transcriptional activation, transcriptional interference, intranuclear transport and so on. They are also closely related to the occurrence, development and prevention of human diseases. Long non-coding RNAs play an important role in the evolution of new proteins, and they could have important functions in cells.
LncRNAs are usually long and have an mRNA-like structure. After splicing, they usually have a 7mC hat at the 5'end and a polyA or no polyA tail at the 3'end. There are dynamic expressions and different splicing modes in the process of differentiation. The expression of lncRNA in different tissues is different, and the expression of lncRNA in the same tissue or organ changes at different growth stages. lncRNAs were found to have different expression patterns in different parts of brain tissue. LncRNAs have many features, such as diversity of regulation: lncRNA can regulate gene expression from chromatin remodeling, transcriptional regulation and post-transcriptional processing. LncRNAs promoters can also bind transcription factors, such as Oct3/4, Nanog, CREB, Sp1, c-myc, Sox 2 and p53. Local chromatin histones also have characteristic modification methods and structural characteristics. Sequence conservativeness of lncRNA is low: only about 12% of lncRNAs can be found in organisms other than humans. Mammalian protein-coding genes account for 1% of total RNA and lncRNAs account for 4% - 9% of total RNA. These lncRNAs are another treasure house for gene function research. At present, many lncRNAs have conserved secondary structures, certain splicing patterns and subcellular localization.
Although more and more lncRNAs have been found, the function of most of them is still unclear. At present, the function of lncRNA is mainly studied by means of lncRNA microarray or next generation sequencing (NGS). Nowadays, the development of gene microarray technology tends to be mature and stable. On this platform, different lncRNA probes can be designed to screen differentially expressed lncRNA and mRNA information in diseases or specific biological processes more quickly using high-throughput methods. The target gene locus of lncRNA can then be found to analyze the regulation mechanism in depth. LncRNA microarrays can quickly and efficiently screen differential lncRNA related to diseases or specific phenotypes, providing theoretical basis for the occurrence, development and prevention of human diseases. CD Genomics' lncRNA microarray covers multiple databases of lncRNA information and a comprehensive sequence of lncRNA. Our lncRNA microarray is designed for human, rat, and mouse. You can also customize the microarrays of other species.
RNA sample requirements
- Purity: OD 260/280 should be 1.9 ~ 2.2 and DNA should be clearly removed;
- Integrity: RNA integrity number (RIN) (≥7) and 28S/18S (≥0.7);
- Concentration: no less than 100ng/µl;
- Amount: no less than 2µg;
- Preservation and transportation: RNA is stored in freezing tubes and transported with dry ice or liquid nitrogen.
CD Genomics offers high-quality services in RNA Microarray. With well-equipped instruments and experienced scientist team, we are dedicated to collaborating with our clients around the world to meet your specific requirements. We have comprehensive capacity and capabilities to provide a broad and integrated portfolio of laboratory and manufacturing services. If you are interested in our services, please contact us for more details.
References
- Wang Q, et al. (2017). "Altered expression of differential gene and lncRNA in the lower thoracic spinal cord on different time courses of experimental obstructive jaundice model accompanied with altered peripheral nociception in rats." Oncotarget, 8(62), 106098-106112.
- Kopp F and Mendell JT. (2018). "Functional Classification and Experimental Dissection of Long Noncoding RNAs." Cell, 172(3), 393-407.
