Peptide-RNA Interaction Microarray Service

As a trusted supplier in the field of microarrays, CD Genomics is committed to designing and developing various effective biological microarray products and providing corresponding testing services. Our microarray detection platform provides a full range of microarray-based analysis services to help our customers reduce costs while using advanced technologies for related research. We are confident to provide you with high-quality products and high-quality services to accelerate the output of research results.

Chromosome Analysis Technology

Microarray technology has been a powerful tool that can be applied to high-throughput and rapid analysis of RNA peptide interactions. Microarray-based ligand screening provides a fast, direct, and high-throughput platform that can identify molecules that bind to RNA. It is a supplement to traditional phenotypic or target-based high-throughput screening methods. Large-scale microarray-based screening is used to identify ligands that bind to "undruggable" protein targets, and can also be used to identify RNA binding agents. Peptide microarrays are also used to measure the dissociation constants of hundreds of RNA peptide complexes. The universal role of RNA in controlling normal physiology and disease makes it an important target for the development of small molecule probes and therapeutic methods.

Fig 1. Strategies for generating microarrays used to screen and profile RNA. (Abulwerdi F A, et al. 2016)

Service

We provide high-quality peptide microarrays based on the analysis of RNA-peptide interactions. RNA plays a key role in most events that occur in cells, and usually has a hairpin (or stem loop) motif as one of its most common secondary structure features. RNA hairpins participate in various biological processes through interaction with RNA-binding proteins, including the regulation of gene expression, as well as mRNA localization and translation. For example, microRNAs that play a role in gene expression regulation often also have hairpin secondary structures. Peptide and peptoid microarrays can be used to identify short RNA hairpin ligands and to identify specific RNA ligands. Our service process includes microarray design (probe synthesis), microarray design and manufacturing, microarray detection, data harvesting and bioinformatics analysis. Both peptide and peptoid probes can be synthesized by combinatorial methods, linking peptides and peptoids to microarray slides. Our microarray service can assist researchers in screening for peptoid libraries that can selectively bind to miRNA precursors and prevent their biogenesis and design microarrays from the reported data. Then use the microarray manufacturing platform to print, and then incubate with the RNA of interest (usually RNA with fluorescent labels). After incubation, the microarray is washed to remove unbound RNA, and a fluorescence scanner is used to detect specific interactions. Finally, signal quantification and statistical analysis are used to identify hits or determine binding constants.

Fig 2.

Technology Benefits

• This method can screen a large number of immobilized ligands at the same time, and has a higher throughput than traditional RNA binding analysis;
• This method requires the use of a relatively small amount of RNA, which makes it particularly powerful when studying RNA that is difficult to prepare in large quantities;
• This method can be performed in parallel or multiple times to generate selective information that cannot be obtained by traditional biochemical analysis;

CD Genomics provides high-quality microarray tools and convenient detection services for gene expression research and RNA-peptide interaction research. We have a large team of scientists with a solid academic foundation and cooperate with researchers in basic biology and biomedical projects from all over the world. The advantage of choosing an analytical testing outsourcing testing service is that there is no need to consider any other costs (such as equipment and laboratory space), and it saves supervision or management. This advanced technology can quickly turn around and provide reliable results, thereby saving valuable time and resources. Please contact us for more detailed information.

References

1. Pai J, Yoon T, Kim N D, et al. High-throughput profiling of peptide–RNA interactions using peptide microarrays[J]. Journal of the American Chemical Society, 2012, 134(46): 19287-19296.
2. Abulwerdi F A, Schneekloth Jr J S. Microarray-based technologies for the discovery of selective, RNA-binding molecules[J]. Methods, 2016, 103: 188-195.