As a high-quality supplier of microarrays, CD Genomics is committed to providing a variety of useful microarray tools and professional testing services for scientific research and non-clinical research. Our microarray testing platform provides a full range of microarray-based mitochondrial transcriptome analysis tools and testing services to help our global customers conduct relevant research and reduce research costs. With our time-tested capabilities and expertise, we are confident to provide you with quality services to escort your research.
Mitochondria exist in most eukaryotic cells and participate in many important biological processes, such as energy metabolism, cell signal transduction, buffering of calcium ion pools, and maintenance of proper redox states. Mitochondrial dysfunction is related to many pathological conditions in humans, which are regulated by the mitochondrial genome (mtDNA) and/or nuclear genes encoding mitochondrial proteins, which affect the occurrence and development of primary and secondary mitochondrial diseases related to mitochondrial dysfunction. Mitochondria are semi-autonomous organelles with their own genomes. Human mtDNA is a circular double-stranded DNA molecule of approximately 16 kb, containing 37 genes, most of which encode tRNA and rRNA required for mitochondrial transcription. Human mitochondria contain more than 1,100 proteins, of which only 13 are encoded by mtDNA. The remaining proteins necessary for mitochondrial functions are encoded in the nuclear genome and synthesized in the cytoplasm and then introduced into the mitochondria. The existence of the unique mitochondrial genome is related to the endosymbiotic origin of this organelle. The latest research on the mitochondrial genome shows that mitochondria evolved from the lineage of proteobacteria. The human mitochondrial genome is one of the first genomes to be fully sequenced, but the molecular mechanism of mtDNA expression still needs a lot of research.
Fig 1. mtDNA structure and mtRNA metabolism. (Kotrys A V, et al. 2020)
We have designed a microarray tool for mitochondrial transcriptome analysis. This tool can be used to analyze known nuclear and mitochondrial genes related to mitochondrial structure and function, as well as genes known to cause mitochondrial dysfunction, such as genes related to energy metabolism. The probe design is based on the public transcriptome database (Genbank) and our own database. We select mitochondrial genes and nuclear genes related to mitochondrial structure and function, energy metabolism, and mitochondrial heritage diseases. Each gene of interest is represented by multiple oligonucleotide probe sets. This tool can be used for transcription analysis of 37 mitochondrial genes, about 500 genes related to energy metabolism, 300 nuclear genes related to mitochondrial genetic diseases, and more than 1,000 nuclear genes related to nuclear mitochondria. We provide one-stop testing and analysis services, from sample pre-processing, microarray testing to data collection and analysis, you can leave it to us with confidence.
Fig 2. The workflow of mitochondrial transcriptomics microarray service.
CD Genomics provides high-quality microarray tools and testing services for mitochondrial transcriptome analysis and research. We have a large and well-equipped team of scientists who are committed to cooperating with researchers from all over the world. If you have relevant testing requirements, please contact us for more detailed information.