Genome-wide DNA Methylation Analysis Service
CD Genomics is a world-leading company that can provide the best solutions for global customers in the field of microarrays. We have successfully completed much scientific research and non-clinical research projects to ensure that we provide the best products and services to customers all over the world.
Genome-wide DNA Methylation Analysis
DNA methylation is a stable, heritable, and reversible epigenetic modification, and it has been shown to be related to almost every biological process. Covalent DNA modification of cytosine at position 5 (5-methylcytosine; 5mC) has become an important epigenetic marker, and it is most commonly found in the environment of CpG dinucleotides in mammalian cells. In pluripotent stem cells and plants, it also exists in non-CpG and CpNpG environments, respectively. DNA methylation can increase the functional complexity of prokaryotic and eukaryotic genomes by providing other ways to control cellular processes. 5mC is of great significance in a variety of biological processes including transcriptional regulation. DNA methylation is also dynamic, so the timing of cellular events can be controlled. Abnormal DNA methylation has been proved to be related to a variety of human diseases, so it is the focus of active research. The methods used to detect DNA methylation have completely changed our understanding of this epigenetic mark and provided new insights into its role in a variety of biological functions.
Fig. 1 Diagram of the TAB-array approach. (Nazor K L, et al. 2014)
Array-based Methylation Profile Analysis
The methylation array service is a service based on bisulfite conversion analysis technology. Our full methylation profile analysis service can quantitatively detect the total methylation level of humans and model animals with a single nucleotide resolution. The measurement range is the full coverage of CpG islands, enhancer regions, open chromatin sites, and other important regions of the methylome. The experimental principle is that after bisulfite treatment, unmethylated cytosine is converted to uracil, while methylated cytosine remains unchanged. Site-specific probes (designed for methylated and unmethylated sites, respectively) are used to interrogate these differentiated loci. Finally, the total methylation level of the interrogated locus is determined by calculating the ratio of the fluorescence signal from the methylated site to the unmethylated site.
Fig. 2 Array-based methylation profile analysis process.
Our Features and Benefits
- Personalized service, a variety of testing content options, you can choose whether to remove the influence of 5hmC;
- Reliable and fast throughput, shorten the detection cycle;
- Data industry-leading data quality, we have a first-class bioinformatics team for data analysis and annotation.
CD Genomics provides high-quality global methylation array screening services. We have a well-equipped experimental platform, a large and professional team of scientists, and we are committed to cooperating with researchers from all over the world to meet the needs of our customers. Customers can contact our employees directly and provide timely feedback on their queries. If you are interested in our one-stop solution service, please contact us for more detailed information.
References
- Nazor K L, Boland M J, Bibikova M, et al. Application of a low cost array-based technique—TAB-Array—for quantifying and mapping both 5mC and 5hmC at single base resolution in human pluripotent stem cells[J]. Genomics, 2014, 104(5): 358-367.
- Gupta R, Nagarajan A, Wajapeyee N. Advances in genome-wide DNA methylation analysis[J]. Biotechniques, 2010, 49(4): iii-xi.
Our products and services are for research purposes ONLY. We do not perform any private testing services that are not for laboratory use.
