CD Genomics is committed to providing microarray solutions for methylation analysis to global customers in the microarray field. We have completed a large number of epigenetic analysis projects in cooperation with many scientific research institutions, universities, companies and non-clinical research organizations.
Epigenetic modifications include DNA methylation changes, histone modifications, and non-coding RNAs (ncRNAs). The transcription of RNA from DNA requires extensive regulation to ensure the correct expression of genes. DNA methylation is the most studied epigenetic mark, including the addition of a methyl group to the 5'position of the DNA cytosine ring. Promoter CpG island hypermethylation is closely related to many pathological changes. mRNA is transcribed from the genomic site of DNA. This process can be regulated by genomic and epigenetic events. However, the completion of the mRNA life cycle includes capping, splicing, and polyadenylation before being exported to the cytoplasm for translation. Correct translation efficiency requires extensive chemical modification (including methylation) of tRNA and rRNA. Histone modifications can change the accessibility of chromatin, thereby changing the affinity for DNA. They include the addition of covalent modifications to the N-terminus of the histone tail, such as lysine acetylation, arginine and lysine methylation, threonine and serine phosphorylation, and lysine threonine and ubiquitination. The emergence of microarray technology can detect a large number of DNA/RNA fragments in a highly parallel manner, which opens up new opportunities for epigenetic research. Many microarray-based techniques for epigenetic analysis are already available.
Fig 1.Schematic outline for methylation-specific oligonucleotide microarray. (Shi H, et al. 2003)
Our methylation analysis service is based on methylation microarray. We will return the trust of customers with excellent product quality, and provide customers all over the world with the most satisfactory products and services. The general process of methylated microarray service includes five items: project customization, protocol confirmation, sample pretreatment, microarray hybridization, and data service. Our project customization includes optional microarray customization services to tailor suitable solutions for customers. At the same time, the necessary sample processing in the current detection strategy includes methylation-sensitive digestion of genomic DNA and target labeling, which is the key to methylation microarray services. Our scientists will monitor your experiment and ensure the quality of every step.
Fig 2. Methylation microarray service process.
|Genome-wide DNA Methylation
|Using microarray hybridization technology, the study of DNA methylation can be extended to the whole genome level. The accelerated development of microarray technology has significantly improved DNA methylation analysis, providing an unprecedented comprehensive view of the DNA methylation landscape. Our genome-wide methylation analysis service provides a combination of methylation microarray products and analytical testing services.
|DNA 5-hmC Profiling
|5hmC is the product of further oxidation of 5mC and is an intermediate of DNA demethylation. Our 5hmC analysis service is based on the hydroxymethylated DNA immunoprecipitation (hMeDIP) method, which can characterize the relative abundance of 5hmC in specific sites or the entire sample genome. Due to the low abundance of 5hmC, the detection of 5hmC is technically more challenging than 5mC. Choose our methylation analysis service, and our team of scientists can help you solve the dilemma of 5hmC analysis.
|DNA 5-foC and 5-caC Profiling
|5fC and 5caC are further oxidized derivatives of 5-hmC, which have potential functions in various cellular processes as more transient intermediates in the DNA demethylation pathway. Our 5fC and 5caC analysis service is based on the MeDIP method to characterize the relative abundance of 5fC and 5caC in a specific site or the entire sample genome.
|mtDNA Methylation Profiling
|With the progress of disease research, the role of the eukaryotic mitochondrial genome in epigenetics is becoming more and more important. mtDNA is smaller than nuclear genome and has a relatively independent system for nuclear genome methylation analysis. Our mtDNA methylation microarray service provides specialized services for mtDNA to analyze the apparent modification of the mitochondrial genome.
|RNA Methylation Profiling
|N6-methyladenosine is the most abundant RNA modification in mammalian mRNA and long-chain non-coding RNA, and it plays an important role in the epigenetic mechanism. We have established a microarray detection method based on methyl-sensitive MazF RNase to analyze methylation in RNA. This method has high specificity and resolution.
CD Genomics provides high-quality commercial, customized and semi-customized microarray products and analysis&testing services based on microarray products. Our microarray test platform has well-equipped laboratories and a team of highly qualified scientists, and we look forward to cooperating with customers all over the world. We have comprehensive design and production capabilities, and can provide a broad and comprehensive combination of laboratory and manufacturing services. If you are interested in our products and services, please contact us for more details