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RNA methylation occurs in different RNA species, including tRNA, rRNA, mRNA, tmRNA, snRNA, snoRNA, miRNA, and viral RNA. As a post-translational modification, RNA methylation plays an important role as an epigenetic mechanism. N6-methyladenosine (m6A) is the most common and abundant methylation modification in RNA molecules present in eukaryotes. RNA methylation can affect the regulation of various biological processes, such as RNA stability and mRNA translation, and abnormal RNA methylation can become the cause of human diseases. Among more than 170 types of RNA modifications, m6A modification is considered to be the most common, reversible, and dynamic eukaryotic mRNA transcription modification, accounting for about 50% of all methylated ribonucleotides. Approximately one-quarter of the transcripts contained m6A modifications, mainly enriched near the stop codon, located in the long internal exons of the 5'and 3'untranslated regions (UTR) of the shared motif RRACH.
Fig 1. The flow of m 6A detected by the two-color microarray. (Li Y, et al. 2015)
In order to facilitate the detection of RNA methylation, CD Genomics has established a series of microarray-based detection methods to analyze base methylation in RNA. The RNase (MazF) that specifically recognizes m6 is used to cleave sample fragments. MazF can specifically cleave the unmethylated (ACA) sequence immediately adjacent to the 5'of the single-stranded RNA, but cannot cleave the methylated sequence. After reverse transcription into cDNA, the digested samples and control samples were labeled with the fluorescence of different colors in 2 and then subjected to microarray analysis. Our microarray design is based on the miCLIP data set, RMbase database, and our own database, providing microarrays of various specifications. A single array is designed with more than 10,000 probes, which can achieve single-base resolution accuracy. Testable samples include humans and laboratory animals.
Fig 2. The m 6A detection process of CD Genomics.
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CD Genomics provides m6A methylation microarray detection services. We have a well-equipped experimental platform, a large and professional team of scientists, and we are committed to cooperating with researchers from all over the world to meet the needs of our customers. Customers can contact our employees directly and provide timely feedback on their queries. If you are interested in our one-stop solution service, please contact us for more detailed information.