As a leading service provider in the field of microarrays, CD Genomics is committed to the design and development of various effective biological microarrays for scientific research and non-clinical research. Our microarray test platform provides a full range of microarray-based piRNA analysis and detection services to help our global customers conduct relevant research and reduce research costs. With our time-tested capabilities and regulatory expertise, we are confident to provide quality services in related testing services to escort your research.
Microarray and /piRNA Expression Profiling
PIWI-interacting RNA (piRNA) is a small single-stranded non-coding RNA, about 26-32 nucleotides in length. piRNA interacts with piwi protein and acts as a complex to regulate cell activity through RNA silencing. piRNA is produced through the main processing pathway and the secondary ping-pong cycle, and comes from three different sources: transposons, protein coding regions and intergenic regions. Similar to miRNAs, they show significant differences in sequence length, expression pattern, abundance and genome organization. Individual piRNAs are very diverse and are poorly conserved even among closely related species. There are thousands of unique piRNA sequences in humans and experimental animals, and they are completely different from microRNAs in terms of length, expression, genome organization, and biogenesis. The most characteristic function of piRNA is to inhibit the activity of transposable factors at the genomic and epigenetic level. In different diseases, the change of piRNA expression may affect the expression of many genes through transcriptional inhibition or epigenetic modification. These affected may participate in many disease-related signal transduction cascades, thereby regulating many cellular events, such as proliferation, apoptosis, migration, invasion.
Fig 1. Biogenic pathways of piRNA in mice. (Sun T, et al. 2019)
Service Process
We have designed piRNA microarrays for humans and experimental animals to evaluate the global expression profile of piRNA. We accept various types of biological samples such as nucleic acids, cells, tissues, and blood. Research objects include but are not limited to humans and laboratory animals. You can also customize it if you have other species of interest. Our service process includes sample reception, quality control, sequence labeling, microarray detection and data analysis. The probe sequence of the microarray comes from the public database (NCBI database) and our own database. The probe is designed as a 60nt antisense oligonucleotide chain. The unique probe design and industry-leading experimental platform enable our piRNA array to analyze the expression of piRNA sensitively and accurately.
Fig 2. piRNA's microarray service process.
Our Features
- Unique design, based on public databases and literature, comprehensive collection and reliable selection of piRNA sequences;
- Reliable and fast throughput process, using uniquely designed oligonucleotide probes to achieve high specificity;
- Repetitive probes of piRNA, high throughput.
CD Genomics provides high-quality microarray tools and testing services for piRNA research. We have a large and well-equipped team of scientists who are committed to cooperating with researchers from all over the world to meet customer requirements. Customers can directly contact our employees and provide timely feedback on their inquiries. If you are interested in our one-stop solution service, please contact us for more detailed information.
References
- Sun T, Han X. The disease-related biological functions of PIWI-interacting RNAs (piRNAs) and underlying molecular mechanisms[J]. ExRNA, 2019, 1(1): 1-16.
- Vella S, Gallo A, Nigro A L, et al. PIWI-interacting RNA (piRNA) signatures in human cardiac progenitor cells[J]. The international journal of biochemistry & cell biology, 2016, 76: 1-11.
- Reeves M E, Firek M, Jliedi A, et al. Identification and characterization of RASSF1C piRNA target genes in lung cancer cells[J]. Oncotarget, 2017, 8(21): 34268.
Our products and services are for research purposes ONLY. We do not perform any private testing services that are not for laboratory use.
