DNA 5-hmC Analysis Service
CD Genomics is a world-leading company that can provide the best solutions for global customers in the field of microarrays. We have successfully completed much scientific research and non-clinical research projects to ensure that we provide the best products and services to customers all over the world.
In the field of epigenetics, in addition to A, C, T, G, and 5-mC, 5-hmC is considered the "sixth base" of the mammalian genome. The abundance of 5-hmC in the genome is 10 to 100 times lower than that of 5-mC. The TET family oxidizes 5mC methyl to hydroxymethyl to form 5hmC. The methylated cytosine in the genome can be modified by the process of DNA demethylation, which will eventually convert the methylated cytosine (5mC) into unmodified cytosine (C). 5hmC is an important intermediate in the demethylation process. DNA demethylation can be performed in one of the following two ways: i. Passive DNA demethylation: due to lack of methylation maintenance enzymes, the frequency of methylated cytosine gradually decreases; ii. Active DNA demethylation: TET The enzyme oxidizes 5mC to an oxidized derivative of 5mC. 5hmC is a new type of cytosine modification with potential regulation and is very popular in the field of epigenetic modification.
Fig 1. Methylation and demethylation in organisms. (Huang Y, et al. 2014)
CD Genomics's 5hmC Testing Service
Similar to the 5mC detection strategy, 5hmC can also be quantitatively analyzed by two methods: immunoprecipitation and chemical modification. The detection process based on MeDIP will not be repeated here. For more information, see our methylation detection service. The chemical strategy can be carried out in several different ways. One is the gold standard-bisulfite conversion method of methylation, which converts 5hmC into CMS, and then combines with the IP method for subsequent detection. The second method is based on the detection of glucosyl modification, which converts 5hmC to g5hmC by β-glucosyltransferase (β-GT), and then performs subsequent detection by JBP1-IP. The third is to convert 5hmC to g5hmC and detect by biotin labeling[3].
Fig 2. CD Genomics's 5hmC testing.
Sample Delivery Requirement
- The minimum DNA concentration required for methylation detection is 2 μg, so whether it is a cell, blood, or tissue sample, the sample volume can not be too small;
- Samples need to be sent at low temperature;
- Multiple samples need to be distinguished, and you need to provide a sample number to distinguish.
The Research Value of 5hmC
Studies have found that the distribution of 5hmC in the genome not only shows general and dynamic characteristics different from 5mC, but also participates in important cell function regulation, involving stem cell pluripotency, cell development and differentiation, and the occurrence and development of cancer. In addition, the distribution and content of 5hmC have begun to be used as one of the important markers for the early diagnosis and prognosis of many cancer diseases.
Our Technology
- High-throughput assay
- Multiple measurement correction
- Excellent data processing team
CD Genomics provides DNA hydroxymethylation microarray screening services. We have a well-equipped experimental platform, a large and professional team of scientists, and we are committed to cooperating with researchers from all over the world to meet the needs of our customers. Customers can contact our employees directly and provide timely feedback on their queries. If you are interested in our one-stop solution service, please contact us for more detailed information.
References
- Huang Y, Rao A. Connections between TET proteins and aberrant DNA modification in cancer[J]. Trends in Genetics, 2014, 30(10): 464-474.
- Shen L, Song C X, He C, et al. Mechanism and function of oxidative reversal of DNA and RNA methylation[J]. Annual review of biochemistry, 2014, 83: 585-614.
Our products and services are for research purposes ONLY. We do not perform any private testing services that are not for laboratory use.
