As a leading service provider in the field of microarrays, CD Genomics is committed to the design and development of various effective biological microarrays for scientific research and non-clinical research. Our microarray test platform provides a full range of microarray-based T-UCR analysis and determination services to help our global customers conduct related research and reduce research costs. With our time-tested capabilities and expertise, we are confident to provide you with quality services to escort your research.
About UCRs and T-UCR
Transcriptional ultra-conserved region (T-UCR) transcript is a new type of lncRNA (UTR-related lncRNAs) transcribed from the ultra-conserved region (UCR). UCR is a subset of conserved sequences located in intragenic and intergenic regions, usually located in vulnerable sites and genomic regions associated with cancer. It is absolutely conserved (100%) between the orthologous regions of the human and rodent genomes, and is a functional element with limited functions. The 481 (>200 bp) sequence with the highest conservation of rodent orthologous fragments in the human genome is defined as UCR. Of these, 56% are non-exons, 23% are partial exons, and 21% may be exons (unconfirmed evidence). Many studies have revealed the hypothetical functions of UCR, such as the antisense regulation of protein-coding genes or other ncRNAs, or the regulation of enhancers. T-UCRs formed by UCR transcription show a ubiquitous and tissue-specific pattern at the expression level. Most importantly, it has been reported that T-UCR changes at the transcription level during human tumorigenesis, indicating that abnormal T-UCR expression profiles can be used to distinguish cancer types.
Fig 1. Re-annotation of UCRs according to their genomic location with respect to protein-coding genes defined by Refseq. (Peng J C, et al. 2013)
Service Process
The T-UCR microarray is designed to analyze the global expression of T-UCRs. The 481 human UCRs have an extra 1kb flanking each end. Our probe adopts a unique design that spans 481 UCRS and its flanking regions to facilitate accurate T-UCR detection and novel T-UCR discovery . Sequence data support comes from our own database and public database. In addition, the array carries specific exon or splice junction probes to reliably and accurately detect about 150 potential T-UCRS. In addition to detecting T-UCR, our T-UCR microarray service also analyzes some lncRNA and mRNA sequences that overlap with UCR in the sense or antisense direction and some genes located in UCRS. We provide full-service T-UCR microarray analysis, from sample preparation to in-depth data analysis. In addition to human UCR and T-UCR expression analysis, we also accept microarray customization services for laboratory animals.
Fig 2. T-UCR microarray service process.
Our Features and Benefits
- The probe sequence coverage is wide, and the probe spanning 481 UCRS and its flanking regions is conducive to the discovery of novel T-UCRS;
- Simultaneous detection of RNA transcription overlapping UCRS and UCR proximal genes is helpful to grasp the functional relationship between T-UCRS and nearby protein coding genes;
- Annotate the relationship between UCR and T-UCR to help discover biomarkers.
CD Genomics provides high-quality microarray tools and testing services for UCR and T-UCR research. We have a large and well-equipped team of scientists who are committed to cooperating with researchers from all over the world. If you are interested in our one-stop solution service, please contact us for more detailed information.
References
- Peng J C, Shen J, Ran Z H. Transcribed ultraconserved region in human cancers[J]. RNA biology, 2013, 10(12): 1771-1777.
- Fabris L, Calin G A. Understanding the genomic ultraconservations: T-UCRs and cancer[J]. International review of cell and molecular biology, 2017, 333: 159-172.
- Qian X X, Peng J C, Xu A T, et al. Noncoding transcribed ultraconserved region (T-UCR) uc. 261 participates in intestinal mucosa barrier damage in Crohn's disease[J]. Inflammatory bowel diseases, 2016, 22(12): 2840-2852.
Our products and services are for research purposes ONLY. We do not perform any private testing services that are not for laboratory use.
