As a leading service provider in the field of microarrays, CD Genomics is committed to the design and development of various effective biological microarrays for scientific research and non-clinical research. Our microarray test platform provides a full range of microarray-based T-UCR analysis and determination services to help our global customers conduct related research and reduce research costs. With our time-tested capabilities and expertise, we are confident to provide you with quality services to escort your research.
Transcriptional ultra-conserved region (T-UCR) transcript is a new type of lncRNA (UTR-related lncRNAs) transcribed from the ultra-conserved region (UCR). UCR is a subset of conserved sequences located in intragenic and intergenic regions, usually located in vulnerable sites and genomic regions associated with cancer. It is absolutely conserved (100%) between the orthologous regions of the human and rodent genomes, and is a functional element with limited functions. The 481 (>200 bp) sequence with the highest conservation of rodent orthologous fragments in the human genome is defined as UCR. Of these, 56% are non-exons, 23% are partial exons, and 21% may be exons (unconfirmed evidence). Many studies have revealed the hypothetical functions of UCR, such as the antisense regulation of protein-coding genes or other ncRNAs, or the regulation of enhancers. T-UCRs formed by UCR transcription show a ubiquitous and tissue-specific pattern at the expression level. Most importantly, it has been reported that T-UCR changes at the transcription level during human tumorigenesis, indicating that abnormal T-UCR expression profiles can be used to distinguish cancer types.
Fig 1. Re-annotation of UCRs according to their genomic location with respect to protein-coding genes defined by Refseq. (Peng J C, et al. 2013)
The T-UCR microarray is designed to analyze the global expression of T-UCRs. The 481 human UCRs have an extra 1kb flanking each end. Our probe adopts a unique design that spans 481 UCRS and its flanking regions to facilitate accurate T-UCR detection and novel T-UCR discovery . Sequence data support comes from our own database and public database. In addition, the array carries specific exon or splice junction probes to reliably and accurately detect about 150 potential T-UCRS. In addition to detecting T-UCR, our T-UCR microarray service also analyzes some lncRNA and mRNA sequences that overlap with UCR in the sense or antisense direction and some genes located in UCRS. We provide full-service T-UCR microarray analysis, from sample preparation to in-depth data analysis. In addition to human UCR and T-UCR expression analysis, we also accept microarray customization services for laboratory animals.
Fig 2. T-UCR microarray service process.
CD Genomics provides high-quality microarray tools and testing services for UCR and T-UCR research. We have a large and well-equipped team of scientists who are committed to cooperating with researchers from all over the world. If you are interested in our one-stop solution service, please contact us for more detailed information.