Research Strategy of lncRNA
Long non-coding RNA (lncRNA) generally refers to RNA molecules that do not have the protein-coding ability and whose transcript length exceeds 200 nt, including intronic/exonic lncRNAs, antisense lncRNAs, overlapping lncRNAs and long intergenic ncRNAs (lincRNAs). LncRNA is closely related to cell cycle and differentiation, development, reproduction, sex regulation, aging and many human diseases. The number of lncRNA is relatively large, about 3-100 times as much as that of mRNA. However, the function of most lncRNA is still unclear. LncRNA is one of the hot topics in biological and medical research. We summarized the main strategies of high throughput research of lncRNA:
- Study the spatiotemporal expression patterns of lncRNA by omics.
Including the types, quantities and expression abundance of lncRNA in biological processes such as different developmental stages and disease progression.
- Functional study of candidate lncRNA.
High throughput screening of control group/treatment group, treatment of different time points and other biologically related differences candidate lncRNAs, and then follow-up functional studies or as a disease treatment target and biomarker research.
- Regulation of biological functions by lncRNA-mRNA or lncRNA-lncRNA Interactions.
For example, genome-scale RNA interactome analysis (RIA-Seq) analysis showed that TINCR lncRNA binds to a variety of mRNAs to regulate cell development.
- miRNA-dependent CeRNA (transcriptome).
- Multi-omics study of lncRNA (genomics, transcriptome, epigenomics).
- lncRNA Encoding Peptide Segment.
These are all about the whole expression of lncRNAs. We listed some common research strategies of lncRNA for reference. You can design an appropriate solution for your research purposes. As a leading service provider in the field of bio-microarray, CD Genomics focuses on designing and developing various and effective RNA microarrays for scientific research and clinical applications. We offer a full range of lncRNA Microarray products and services to help our worldwide customers shorten the microarray designing time and lower the cost of research. With our proven competencies and regulatory expertise, we are therefore confident in designing, developing, testing, and manufacturing of numerous lncRNA microarrays to suit your special needs in any biological and clinical projects.
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References
- Zhan S, et al. (2016). "Genome-wide identification and characterization of long non-coding RNAs in developmental skeletal muscle of fetal goat." Bmc Genomics, 17(1), 666.
- Yang B, et al. (2016). "Distinct Hippocampal Expression Profiles of Long Non-coding RNAs in an Alzheimer's Disease Model." Molecular Neurobiology, 1-14.