CD Genomics is a world-leading company that can provide the best solutions for researchers in the field of microarrays in the field of biomedicine. We have extensive project experience in microarray production and testing and can ensure that we provide the best products and services to customers around the world.
Aptamers are highly structured oligonucleotides with specific binding properties, which are selected from a combinatorial library of synthetic nucleic acids by an iterative process referred to as Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Aptamers have many advantages because they can compete with antibodies to assist in multiple analysis methods for target and non-target molecular analysis, such as biosensors, affinity capillary electrophoresis, and affinity chromatography. They have high applicability in the identification, separation and detection of various analytes, such as amino acids, drugs, metal ions, peptides, proteins and even cells. The characterization of the binding affinity and specificity of aptamer candidates is a key but challenging step in the discovery process because the throughput of traditional tools is usually low. However, by performing these assays on a microarray, characterization can be performed in a high-throughput parallel manner.
Aptamer microarray technology overcomes the limitations of protein/antibody microarrays due to antibodies and takes advantage of the advantages of DNA/RNA microarrays. Aptamers are fixed on the surface of the microarray and serve as probes for various substances. The aptamer microarray manufacturing technology can be divided into two categories. On the one hand, aptamers can be synthesized directly on the surface of the microarray through in-situ synthesis. The limitation of the in-situ synthesis of ready-to-use microarray is that it is impossible to purify the aptamer after synthesis. On the other hand, aptamers can be attached to the microarray after synthesis and purification, the so-called post-synthesis immobilization.
Fig.1. Schematic overview over different aptamer microarray formats. (Witt M, et al. 2015)
Our DNA/RNA aptamer microarray service is a customized microarray service, customizing microarray products with suitable specifications and probe sets for customers, and then completing subsequent detection and analysis work through our microarray test platform.
Microarray customization services are optional semi-custom and fully customized microarrays. The service process includes microarray design & fabrication, sample pre-processing, microarray detection and data analysis. Our aptamer binding assay process includes assistance in sequence design, standard or custom DNA/RNA (2'-OMe) aptamer microarray synthesis, and the use of binding molecules (such as proteins, nucleic acids, carbohydrates or other types) provided by you Small molecules) for binding analysis. In the detection stage, single-color or dual-color detection is used and quantitative measurement using a combination of appropriate methods, image data processing, and signal data processing. After receiving your sample, we will complete the entire analysis process within 15-30 days and provide you with a detailed analysis report, including data results, array representative and original images, and original data.
Just send your sample to us-we will screen or optimize its combination with the aptamer sequence.
In addition, our customized service can provide assistance for custom aptamer sequence design, and in-situ synthesis of customer-specified aptamer sequences on addressable high-density microarrays.
Fig 2. Aptamer microarray service process.
(1) Aptamers are much smaller than antibodies and can form a higher density affinity stationary phase on the surface of the microchannel;
(2) Aptamers are easier to be modified at the terminal sites and have various functional groups, which makes them easier to fix on the support;
(3) Aptamers are more stable than antibodies at room temperature and under harsh elution conditions (such as extreme pH, urea, organic solvents, chaotropic salts or other detergents);
CD Genomics provides analysis services based on aptamer microarrays. We have an advanced microarray experiment platform, a professional team of scientists, and are committed to cooperating with researchers from all over the world. Customers can directly contact our employees and provide timely feedback on their questions. If you want to save valuable time and resources, please contact us for more detailed information.